MiRNA mimic screen for improved expression of functional neurotensin receptor from HEK 293 cells
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چکیده
منابع مشابه
A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization
The human embryonic kidney 293 (HEK-293) cells are commonly used as host for the heterologous expression of membrane proteins not least because they have a high transfection efficiency and faithfully translate and process proteins. In addition, their cell size, morphology and division rate, and low expression of native channels are traits that are particularly attractive for current-voltage mea...
متن کاملThe ethics of HEK 293.
1 Congregation for the Doctrine of the Faith, Donum vitae (February 2, 1987), Introduction, 5. 2 Ibid., I, 1. The topic of embryonic stem cells is very much in the forefront of public interest. The moral stance on obtaining stem cells from embryos is clear: The Church has always taught that all human life from the very beginning must be respected and protected. If human embryos are killed to ob...
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Background: Understanding of cellular and molecular mechanisms involved in cell/tumor growth and progression has led to molecular-targeted therapy. HER1 and HER2 are two main oncogenic components of HER-receptor family considered as targets for cancer research and therapy. Reliable results of HER- related research and targeted therapies dependent upon cell line with known HER expression status....
متن کاملCharacterization of a Mutant Dopamine Transporter in HEK-293 Cells
................................................. 3 Background.............................................. 4 Materials and Methods...................................8 Results....................................................12 Discussion................................................14 Acknowledgments.......................................16 References.........................................
متن کاملInteraction of neuronal nitric oxide synthase with alpha1-adrenergic receptor subtypes in transfected HEK-293 cells
BACKGROUND The C-terminal four amino acids (GEEV) of human alpha1A-adrenergic receptors (ARs) have been reported to interact with the PDZ domain of neuronal nitric oxide synthase (nNOS) in a yeast two-hybrid system. The other two alpha1-AR subtypes have no sequence homology in this region, raising the possibility of subtype-specific protein-protein interactions. RESULTS We used co-immunopreci...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2015
ISSN: 0006-3592
DOI: 10.1002/bit.25567